My first stop at FRIM on a dull quiet morning was at the HR department, where I and my fellow interns were given the relevant documents to fill up and hand in. Then I headed to the Natural Products division laboratory, where I was supposed to meet my supervisor.
Unfortunately, she was on leave, but she gave some directions to me, which was to read some of the journals (co-authored by her) and manual on column chromatography and TLC methods.
Since reading can be done at a later time, I chose to observe my fellow interns as they did their work while being instructed by their supervisors. There were two groups I obseved:
1st:
Isolation of organic compounds by soaking raw materials in organic solvent first and then do claisen-schmidt condensation with rotary evaporator, and then compound isolation and identification with TLC
2nd:
Weighing of 32 different plant raw materials, before soaking overnight in solvent before compound isolation
In short, mostly learnt about isolation of compound and TLC method. Also introduced to HPLC method and column chromatography.
Given a trip around the laboratories and introduced to many analytical machines such as HPLC, FTIR, etc
Homework: read journals and get to know different stationary eluents for column chromatography, and know the principles behind HPLC.
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